Articles

 

Friday
Jan062012

Veterinary Homeopathy Research

Homeopathic prescribing for chronic conditions in equine veterinary practice in the UK

Mathie, R., Baitson, E., Hansen, L., Elliott, M., Hoare, J.

Veterinary Record 2010;166:234-237: Journal of the British Veterinary Association  

Abstract

Twelve Faculty of Homeopathy veterinarians recorded data systematically at 777 consecutive homeopathic appointments for horses over a period of 12 months. A spreadsheet enabled the recording of information, which included the date of appointment; horse and owner identity (anonymised); sex of horse; main medical problem treated; whether the condition was chronic or acute; whether the appointment was new or a follow-up; owner-assessed clinical outcome on a seven-point scale, ranging from -3 to +3, compared with the first appointment; homeopathic medicine(s) prescribed; and whether any conventional or other complementary/alternative medicine (CAM) was being used concurrently to treat the condition. Data from 289 horses comprised a total of 305 individual conditions identified as chronic in nature, of which 234 had a follow-up assessment. At the final appointment for chronic cases during the study period, 4.3 per cent were receiving conventional medication and 17.1 per cent were being given another CAM treatment in addition to homeopathy. The eight chronic conditions most frequently treated with homeopathy were: arthritis, headshaking, laminitis, chronic obstructive pulmonary disease, sweet itch, dermatitis, sarcoidosis and Cushing's syndrome.

 

 

Sunday
Jan012012

Love of Animals Led To Language and Man's Domination of Earth

When our apemen ancestors began to interact with animals they developed empathy and the ability to communicate, claims anthropologist Pat Shipman.

Humans became masters of the planet for a startling reason: our love of animals gave us unsurpassed power over nature. This is the claim of a leading American anthropologist who says our prehistoric ancestors' intense relationships with other creatures – including those we hunt, keep as pets and use for food – propelled humanity towards global domination. Interacting with animals on an intimate basis led humans to develop sophisticated tools and evolve enhanced communication skills, including language itself, Dr Pat Shipman of Pennsylvania State University told the Observer. Animals also taught us that others – even other species – have emotions, needs and thoughts, while they also helped us to evolve the vital skills of empathy, understanding and compromise. "The longest and enduring trend in human evolution has been a gradual intensification of our involvement with animals," she added. "But now our world is becoming increasingly urbanised and we are having less and less contact with them. The consequences are potentially catastrophic."

Shipman traces humanity's animal connection to the period 2.5 million years ago when our hominid ancestors first made tools. These crafted pieces of stone still litter sites in eastern Africa, including the Olduvai Gorge in Kenya, and bear testimony to the mental transformation in our ancestors' brains. "These apemen didn't just pick up stones and use them to hammer or pound prey or plants," said Shipman. "They shaped those rocks for specific purposes. They had a mental image of the kind of tools they needed and created them by chipping away at a large piece of stone until they got what they wanted." And what they wanted were tools for cutting up carcasses. In other words, the sharp stone flakes spread over Olduvai were not used primarily as weapons to kill animals or to hack down plants, but to process dead animals that had already been brought down by other carnivores. Apemen had begun to scavenge for meat from carcasses of prey killed by leopards, cheetahs and other carnivores. Armed with sharp blades, they could cut off chunks of antelope or deer and escape quickly before being eaten themselves by an enraged lion, they discovered.

And that was the crucial point that began our special relationship with the animal kingdom, said Shipman, whose book, The Animal Connection, is published this week. "Until that point, we had been a prey species. Carnivores ate us. Then we began scavenging before going on to hunt on our own behalf. Meat provided our ancestors with a wonderful, rich source of sustenance. However, scavenging for it left us in a very vulnerable position. We were still just as likely to be consumed when confronted by a carnivore as we were to kill in our own right. To survive, we had to learn about the behaviour of a vast number of different species – the ones we wanted to kill and the ones we wanted to avoid. "For example, we would have learned to spot when lions were preparing to mate – when a male was showing off to a female – so that we could take some its prey while it was otherwise occupied. We would have also built up knowledge about the migration of species such as wildebeest and other animals." In the end, this expertise would have become crucial to human survival, a point illustrated in the cave paintings in Lascaux and Chauvet in France and the other caves painted by humans 20,000 to 30,000 years ago. They show us that after 2 million years of evolution, humans had become utterly fixated by animals.

"These paintings are stunningly beautiful and superbly crafted," said Shipman. "Sometimes scaffolding was erected in the caves. At the same time, artists went to enormous lengths to get their pigments mixed with the right binding agents and placed in exactly the right spot. And what did they depict when they got things just right? Animals, animals and more animals. "There are no landscapes and only a handful of poorly executed depictions of humans. By contrast the paintings of lions, stags, horses, bulls and the rest are magnificent. We were besotted with animals because our lives depended on our relationships with them." Not long after these paintings were created, the first animal – the dog – was domesticated, followed some time later by the horse, sheep, goat and others. The development was crucial. In each case, humans had to learn to put themselves in the minds of these creatures in order to get them to do our bidding. In this way our senses of empathy and understanding, both with animals and with members of own species, were enhanced.

Our special relationship with animals is revealed today through our desire to have pets. "Humans are the only species on Earth to have one-to-one relationships with a member of another species," said Shipman. "No other creature would waste resources on a member of another family, let alone a member of another species. But we do and that is because we have evolved such close ties with specific animals over the millennia and because we are adapted to empathise with other creatures. It is a unique human attribute. We get so much from animals, much more than we appreciate."

Unfortunately, as society becomes increasingly urbanised those ties are being stretched and broken, added Shipman. "Our links to the animal world are precious and shouldn't be taken for granted," she said.

Robin McKie, The Observer, 2.10.11

 

Tuesday
Dec062011

Co-supplementation with vitamin E and coenzyme Q10 reduces circulating markers of inflammation in baboons 

Background—Inflammation and oxidative stress are processes that mark early metabolic abnormalities in vascular diseases.

Objectives—We explored the effects of a high-fat, high-cholesterol (HFHC) diet on vascular responses in baboons and the potential response-attenuating effects of vitamin E and coenzyme Q10 (CoQ10) supplementation.

Design—We used a longitudinal design by subjecting 21 baboons (Papio hamadryas) to sequential dietary challenges.

Results—After being maintained for 3 months on a baseline diet (low in fat and cholesterol), 21 baboons were challenged with an HFHC diet for 7 wk. The serum C-reactive protein (CRP) concentrations did not change. Subsequent supplementation of the HFHC diet with the antioxidant vitamin E (250, 500, or 1000 IU/kg diet) for 2 wks reduced serum CRP concentrations from 0.91 ± 0.02 to 0.43 ± 0.06 mg/dL. Additional supplementation with CoQ10 (2 g/kg diet) further reduced serum CRP to ≈30% of baseline (0.28 ± 0.03 mg/dL; P = 0.036 compared with the HFHC diet). Introduction of the HFHC diet itself significantly decreased serum P-selectin (from 48.8 ± 7.2 to 32.9 ± 3.7 ng/dL, P = 0.02) and von Willebrand factor (from 187.0 ± 10.1 to 161.9 ± 9.0%, P = 0.02) concentrations. However, neither vitamin E alone nor vitamin E plus CoQ10 significantly altered the serum concentrations of P-selectin or von Willebrand factor.  

Conclusion: Dietary supplementation with vitamin E alone reduces the baseline inflammatory status that is indicated by the CRP concentration in healthy adult baboons. Co-supplementation with CoQ10, however, significantly enhances this anti-inflammatory effect of vitamin E.

Reference: Wang., Xing Li., David L Rainwater, Michael C Mahaney & Roland Stocker

Cosupplementation with vitamin E and coenzyme Q10 reduces circulating markers of inflammation in baboons. Am J Clin Nutr. 2004 September ; 80(3): 649–655.

Thursday
Nov172011

Effect of Aloe vera on nitric oxide production by macrophages during inflammation.

Inflammation is generally considered as an essentially protective response to tissue injury caused by noxious physical, chemical or microbiological stimulus. It is a complex process involving various mediators, such as prostaglandins, leukotrienes and platelet activating factor. The major macrophage derived inflammatory mediators such as pro-inflammatory cytokines, tumour necrosis factor-a (TNF- a) and the reactive free radical nitric oxide (NO) synthesized by inducible NO synthase (iNOS), contribute to the development of inflammatory diseases. Thus, inhibition of the excessive production of TNF-a and/or NO could be employed as criteria to evaluate potential antiinflammatory compounds. The current management of inflammatory diseases is limited to the use of antiinflammatory drugs whose chronic administration is associated with several adverse effects. Plant-derived products are slowly emerging as a viable alternative because they are cheap, abundantly available and relatively less toxic.

The genus Aloe belongs to the Asphodelaceae family, Alooideae subfamily, and comprises about 420 species of succulent plants. They are indigenous to southern and eastern Africa and Madagascar but have been introduced in other tropical countries, the Mediterranean area and the West Indies. Aloe has been used as a folk medicine for 3000 years and in the last decade Aloe vera is being extensively used in health drinks, topical creams, toiletries and cosmetics. From the leaves of Aloe plants, three types of commercial products are obtained namely, the dried exudate, excreted from the aloin cells present in the zone of the vascular bundle, the gel, a mucilaginous juice present in the centre of the leaf and the oil, extracted by organic solvents. The dried exudate is used as a bitter in alcoholic beverages while the oil, comprising the fatty fraction of the leaf is used primarily in the cosmetic industry as a pigment carrier and soothing agent. Studies have shown that the gel component has antiinflammatory activity that is mediated through antibradykinin activity and inhibition of prostaglandin production. However, no studies have been undertaken with regard to the antiinflammatory activity of the leaf exudate. Accordingly, in this study, we have evaluated the antiinflammatory potential of Aloe vera leaf exudate as also demonstrated that this antiinflammatory activity is mediated partly via reduction of nitric oxide production in macrophages.

Research trial

OBJECTIVE: To demonstrate the mechanism of action mediating the acute and chronic antiinflammatory activity of leafy exudate of Aloe vera (AVL) in animal models of inflammation.

MATERIALS AND METHODS: The acute antiinflammatory activity of AVL was evaluated using carrageenan and dextran as phlogistic agents while its chronic antiinflammatory effect was investigated in a complete Freund's adjuvant-induced model of arthritis. The degree of inflammation in all models was measured plethysmographically. The effect of AVL on nitric oxide production in mouse peritoneal macrophages was measured by the Griess reagent.

RESULTS: AVL (25 mg/kg) significantly reduced carrageenan and dextran-induced pedal edema in rats by 61.9% and 61.7%, respectively. In the Freund's adjuvant-induced model of chronic inflammation, AVL showed chronic antiinflammatory activity but failed to decrease the arthritic index indicating the absence of antiarthritic activity. AVL (10 [micro]g/ml) caused a decrease in NO production in macrophages without causing toxicity.

CONCLUSION : AVL possesses acute and chronic antiinflammatory activity, which is partly mediated by reduced production of NO, which in turn prevents the release of inflammatory mediators.

Reference: D. Sarkar, A. Dutta, M. Das, K. Sarkar, C. Mandal, M. Chatterjee. Indian Journal of Pharmacology 37.(Nov-Dec 2005).

 

 

 

Thursday
Nov172011

Fibrosarcoma with Typical Features of Postinjection Sarcoma at Site of Microchip Implant in a Dog: Histologic and Immunohistochemical Study 

Abstract

A 9-year-old, male French Bulldog was examined for a subcutaneous mass located at the site of a microchip implant. Cytologic examination of the mass was suggestive of a malignant mesenchymal neoplasm. Histologically, the mass was confirmed as a high-grade infiltrative fibrosarcoma, with multifocal necrosis and peripheral lymphoid aggregates. By immunohistochemistry, the sample was investigated for vimentin, smooth-muscle actin (SMA), CD3, CD79α, and CD18. All the neoplastic cells were positive for vimentin. Scattered cells at the periphery of the lesion were also positive for SMA, highlighting a myofibroblastic phenotype. The lymphoid cells were positive for CD18 and CD3. No aluminum deposits were detected by the aurintricarboxylic acid method. A diagnosis of fibrosarcoma morphologically similar to feline postinjection sarcomas was made. Fibrosarcomas at the site of injections have been reported in dogs and ferrets. Furthermore, neoplastic growth at the site of microchip implant in dog and laboratory rodents has been described.

Background

On April 2004, Leon, a 9-year-old male French Bulldog, was examined by the referring veterinarian, based in Guelph, Ontario (Canada), for the sudden growth of a subcutaneous 3 × 3-cm mass located on the dorsal midline of the neck, just cranial to the shoulders. The dog was regularly vaccinated against the most common canine infectious diseases and rabies, and was microchipped (Indexel, Merial, Lyon, France) in September 2003.

A complete physical examination excluded any further alteration. Fine needle aspiration of the mass highlighted a single population of large, bipolar streaming spindle cells in swirling bundles. Cells had moderate nuclear/cytoplasm ratios and oval nuclei, with fine chromatin, multiple dark nucleoli, and prominent anisocytosis and anisokaryosis. Mitotic figures were rare, and no extracellular matrix was present. No evidence of inflammation or sepsis was observed. The cytologic diagnosis was fibrosarcoma.

On the veterinarian's advice, the owner decided to have the mass removed. The mass was surgically excised with 2 cm of margins. The microchip, which was detected attached to the mass, was also removed. The tissue was immediately fixed in 10% neutral buffered formalin, routinely processed, and paraffin embedded for histologic examination.

Reference: Marta Vascellari, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universitàa 10, 35020 Legnaro (PD) (Italy).